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Pro-democracy protesters in Hong Kong have taken down a Chinese national flag and thrown it into the city's harbour.

In the latest show of defiance, tens of thousands of demonstrators put up barricades, blocking the entrances to a major tunnel and seriously obstructing traffic.

The rally walked past an end-point agreed with police and marched into Mong Kok, a shopping district popular with tourists, leading to Victoria Harbour.

In a move likely to inflame Beijing, a protester climbed up a flagpole and removed the Chinese standard before it was flung into the water.

Sky's chief correspondent Stuart Ramsay watched protesters blocking the entrance to the main subway tunnel linking Kowloon and Hong Kong island.

"This is unheard of," he said. "It has never been seen before.

"There's youths everywhere building barricades. They're not being stopped. The police haven't been present at all all day."

Noting that the protesters were "really well organised," he added that "everything is done beneath umbrellas to protect their identities."

In another part of Kowloon, police fired tear gas at protesters after, they said, a large group sprayed grafitti outside the Tsim Sha Tsui police station, set fire to piles of rubbish and damaged vehicles inside the station.

In a statement, police said they threw bricks and hard objects into the building, causing "serious public danger".

Tear gas was also seen in other parts of the city as the night drew on, as officers belatedly intervened to combat the protest.

The demonstrations were initially triggered by a bill, since suspended, that would allow extraditions to mainland China.

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But they have become a wider test of the Hong Kong government's commitment to democracy.

Protesters are also demanding independent inquiries into police use of force, the resignation of Hong Kong leader Carrie Lam, and full democratic reform.